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In brief, the volunteers were included in the study in successive groups of three during the fall of.
The volunteers had fasted overnight before orally ingesting the dose at 11:00 a. Stool consistency was graded on a scale from 1 to 5, where the grades seen in this study were 1 and 2 firm and soft formed stools, respectively and 3 viscous opaque liquid or semiliquid which assumes the shape of the container.
None of the volunteers became ill enough to warrant earlier treatment. Microbiological detection of ETEC Stool specimens were acquired daily starting on the first day after the dose had been ingested.
Stool DNA purification For this study, we designed a DNA purification method that enables extracting genomic DNA equally well from all stool microbes and minimizing the amount of co-purified PCR inhibitors while allowing for purification in a microplate format.
Up to one stool specimen for each day from each volunteer was included in the study, including the day the volunteers ingested the dose.
Note that the integrity of the aluminum foil seal should be checked before vortexing, and that a fresh foil should be added after adding the ammonium acetate.
After incubation, the filtrates were collected by centrifugation into a 1.
The qPCR primers were designed to target the E. Gene sequences from 37 E.